Device

Part:BBa_K4472998:Design

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-10)


Ribozyme half 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoters of split ribozyme half 1 and split ribozyme half 2 need to be different in order to get them synthesized. If they are the same there is too much repetition for the synthesis companies.


Source

The original ribozmye is from Tetrahymena thermophila.

References

Gambill L, Staubus A, Ameruoso A, Chappell J. A split ribozyme that links detection of a native RNA to orthogonal protein outputs. bioRxiv. January 2022:2022.01.12.476080. doi:10.1101/2022.01.12.476080